Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

Data Example

Western BlotView Larger

Detection of Human HSP90 alphaby Western Blot.Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human HSP90a Antigen Affinity-purified Polyclonal Antibody (Catalog # A-440) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP90a at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Background: HSP90 alpha

The heat shock protein-90 kDa (HSP90) is a composite name for a large group of genes whose molecular weights average 90 kDa. HSP90 functions primarily as a molecular chaperone, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. In addition to its role as a molecular chaperone and stress response protein, HSP90 is a central component in a number of cellular processes including hormone signaling and cell cycle control. HSP90 is ubiquitously expressed, highly conserved, and accounts for 1-2% of the total cellular protein. Eukaryotic cells have two principal forms of HSP90 including the inducible HSP90 alpha(also known as HSP90AA1, HSP90A, HSPC1, HSPCA and HSP86) and the constitutively expressed HSP90 beta(also known as HSP90AB1, HSP90B, HSPCB, HSPC2, and HSP89-beta. While HSP90 alphaand HSP90 betashare 90% identity, this affinity-purified polyclonal antibody is specific for HSP90 alpha.

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Related Research Areas

• Diabetic Peripheral Neuropathy
• Heat Shock Proteins (HSPs)
• Neurofibrillary Tangle Formation