Recombinant Human His6-ATG3 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-670
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v),1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: ATG3
Autophagy-related 3 (ATG3), also known as Apg3L and Apg3p, functions as a regulatory component of autophagosome biogenesis necessary for autophagy. It is a 314 amino acid (aa) protein with a predicted molecular weight of 36 kDa. ATG3 exhibits 98% aa sequence identity with both its mouse and rat orthologs. It is a Ubiquitin-conjugating (E2)-like enzyme that works in concert with Atg12-Atg5, a Ubiquitin Ligase (E3)-like enzyme conjugate, to catalyze the transfer of Atg8 (LC3, GATE-16, and GABARAP in mammals) to phosphatidylethanolamine (PE) in the developing autophagosome membrane (1-4). This lipidation of Atg8 proteins is necessary for autophagosome maturation (5).
- Ichimura, Y. et al. (2000) Nature 408:488.
- Ichimura, T. et al. (2004) J. Biol. Chem. 279:40584.
- Yamaguchi, M. et al. (2010) J. Biol. Chem. 285:29599.
- Otomo, C. et al. (2013) Nat. Struct. Mol. Biol. 20:59.
- Shpilka, T. et al. (2012) Genome Biol. 12:226.
FAQs
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