Recombinant Human Tetra-Ub (K63-linked) Rhodamine 110, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UC-355
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.0, 50 mM NaCl |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Tetra-Ubiquitin
Linkage specific poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB"s") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules.K63-linked tetra-Ubiquitin chains are manufactured using recombinant wild-type human Ubiquitin and linkage-specific enzymes.The use of purely enzymatic techniques avoids the potential for contaminating synthetic intermediates.This fluorogenic substrate is intended for use with deubiquitinating enzymes that are unable to use mono-Ubiquitin-Rh110 (Catalog#U-555) as a substrate, or enzymes with activity that is stimulated by poly-Ubiquitin chains. The substrate consists of a K63-linked tetra-Ubiquitin chain with a single rhodamine attached to the C-terminus of the proximal Ubiquitin.
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