Recombinant Human His6-VCIP135/VCPIP1 Protein, CF Summary
Reaction conditions will need to be optimized for each specific application. We recommend an initialVCIP135concentration of 100-500 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555) or Poly-ubiquitin chain substrates.
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-590
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: VCIP135/VCPIP1
Valosin-containing protein p97/p47 complex-interacting protein, or VCIP135, is a member of the OTU superfamily of Deubiquitinating Enzymes. It is 1222 amino acids (aa) in length with a predicted molecular weight of 134 kDa. Human VCIP135 shares 95% aa sequence identity with the mouse ortholog. In the cell, VCIP135 function requires membrane association and interaction with p97, both of which are inhibited by VCIP135 phosphorylation during mitosis. VCIP135 activity is necessary for VCP-mediated reassembly of Golgi stacks after mitosis. In vitro, VCIP135 efficiently hydrolyzes K11- and K48-linked Poly-ubiquitin chains.
- Zhang, X. et al. (2014) Cell Sci 127: 172-181
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