Recombinant Human NEDD8 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UL-812
| Formulation | Xmg/ml (X μM) in 50 mM Hepes, pH 8, 150mM NaCl, and 1mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: NEDD8
Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one Ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide (1,2). Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat, and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits (3). Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes (4). Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates (5). For example, transfer of NEDD8 to the Cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of Ubiquitin ligases (E3) (6-8). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival (9,10). Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies (11,12).
- 1. Kumar, S. et al. (1993) Biochem. Biophys. Res. Commun. 195:393. 2. Kamitani, T. et al. (1997) J. Biol. Chem. 272:28557.
- 3. Huang, D.T. & B.A. Schulman (2005) Methods Enzymol. 398:9.
- 4. Gong, L. & E.T. Yeh (1999) J. Biol. Chem. 274:12036.
- 5. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
- 6. Petroski, M.D. & R.T. Deshares (2005) Nat. Rev. Mol. Cell Biol. 6:9.
- 7. Hjerpe, R. et al. (2012) Biochem. J. 441:927.
- 8. Calabrese, M.F. et al. (2011) Nat. Struct. Mol. Biol. 18:947.
- 9. Tateishi, K. et al. (2001) J. Cell. Biol. 155:571.
- 10. Ohh, M. et al. (2002) EMBO Rep. 3:177.
- 11. Luo, Z. et al. (2012) Cancer Res. 72:3360.
- 12. Soucy, T.A. et al. (2010) Genes Cancer 1:708.
Citations for Recombinant Human NEDD8 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein ExpressionAuthors: X Liu, JM Reitsma, JL Mamrosh, Y Zhang, R Straube, RJ DeshaiesMol. Cell, 2018;69(5):773-786.e6.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylationAuthors: H Zhou, J Lu, L Liu, D Bernard, CY Yang, E Fernandez-, K Chinnaswam, S Layton, J Stuckey, Q Yu, W Zhou, Z Pan, Y Sun, S WangNat Commun, 2017;8(1):1150.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as afeedback regulator of biochemical and oncogenic activity.Authors: Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, Rechler W, Hao B, Ramanathan Y, Singh BJ Biol Chem, 2015;290(1):296-309.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G.Authors: Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, Cartozo N, Li M, Jager S, Mason-Herr J, Hayashi F, Yokoyama S, Krogan N, Harris R, Peterlin B, Gross JPLoS Pathog, 2012;8(12):e1003085.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
FAQs
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