Human 26S Proteasome Protein, CF Summary
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Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-365
| Formulation | Xmg/ml (X μM) in 20 mM HEPES pH 7.5, 20 mM NaCl, 2 mM Mg-ATP, 15% (v/v) Glycerol |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: 26S Proteasome
The 26S Proteasome is the major non-lysosomal protease in eukaryotic cells and is responsible for the degradation of ubiquitinated substrates and misfolded proteins. It is composed of two subcomplexes: the 20S Proteasome core particle and the 19S Proteasome regulatory particle. The 20S Proteasome facilitates proteolytic cleavage of protein substrates and is composed of 28 subunits arranged into four stacked rings (1,2). The outer rings of the 20S Proteasome are composed of seven related but non-identical, non-catalytic subunits, alpha1-7, that form a gate and restrict substrate access. The inner rings of the 20S Proteasome are composed of seven related but non-identical subunits, beta1-7. Beta1, 2, and 5 have proteyolytic activity. The 19S Proteasome caps one or both ends of the core particle and regulates substrate access to the catalytic core in an ATP-dependent manner by modulating 20S Proteasome conformation (3-5). The 19S Proteasome consists of a base subcomplex and a lid subcomplex. The base subcomplex is composed of six AAA+ family members, scaffolding proteins, and regulatory proteins involved in Ubiquitin recognition (2,6). The 19S Proteasome lid subcomplex contains eight subunits plus one deubiquitinating enzyme, Rpn11 (2,6). Small molecules that inhibit the activity of the 26S Proteasome are used to study the function of short-lived intracellular proteins and for the clinical treatment of certain forms of cancer (7). This highly purified 26S Proteasome preparation (from the transformed HEK cell line) can be used in vitro for the degradation of peptide substrates and poly-ubiquitinated proteins.
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Citations for Human 26S Proteasome Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- A new gold(I) complex-Au(PPh3)PT is a deubiquitinase inhibitor and inhibits tumor growthAuthors: X Li, Q Huang, H Long, P Zhang, H Su, J LiuEBioMedicine, 2018;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- MPSR1 is a cytoplasmic PQC E3 ligase for eliminating emergent misfolded proteins in Arabidopsis thalianaAuthors: JH Kim, SK Cho, TR Oh, MY Ryu, SW Yang, WT KimProc. Natl. Acad. Sci. U.S.A., 2017;114(46):E10009-E10017.Applications: Bioassay
- Diabetogenic agent alloxan is a proteasome inhibitorAuthors: W Zhou, L Wei, T Xiao, C Lai, M Peng, L Xu, X Luo, S Deng, F ZhangBiochem. Biophys. Res. Commun., 2017;0(0):.Applications: Bioassay
- Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB(+1)Authors: Michal ChojnackiFEBS Lett, 2016;590(24):4573-4585.Applications: Bioassay
- Proteasome addiction defined in Ewing's sarcoma is effectively targeted by a novel class of 19S proteasome inhibitorsAuthors: Neerav N ShuklaCancer Res, 2016;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signallingAuthors: BJ Yang, XX Han, LL Yin, MQ Xing, ZH Xu, HW XueNat Commun, 2016;7(0):11388.Species: HumanSample Types: Recombinant ProteinApplications: Enzyme Assay
- Proteasome inhibition and oxidative reactions disrupt cellular homeostasis duringheme stress.Authors: Vallelian F, Deuel J, Opitz L, Schaer C, Puglia M, Lonn M, Engelsberger W, Schauer S, Karnaukhova E, Spahn D, Stocker R, Buehler P, Schaer DCell Death Differ, 2015;22(4):597-611.Species: N/ASample Types: ChipApplications: Bioassay
- RedOx status, proteasome and APEH: insights into anticancer mechanisms oft10,c12-conjugated linoleic acid isomer on A375 melanoma cells.Authors: Bergamo P, Cocca E, Palumbo R, Gogliettino M, Rossi M, Palmieri GPLoS ONE, 2013;8(11):e80900.Species: HumanSample Types: Whole CellsApplications: Bioassay
- ERalpha phosphorylation at Y537 by Src triggers E6-AP-ERalpha binding, ERalphaubiquitylation, promoter occupancy, and target gene expression.Authors: Sun J, Zhou W, Kaliappan K, Nawaz Z, Slingerland JMol Endocrinol, 2012;26(9):1567-77.Species: HumanSample Types: ProteinApplications: Bioassay
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