Recombinant Human GABARAP Agarose Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UL-415
| Formulation | 0.5 ml of GABARAP agarose is supplied in a 1 ml total volume of 100 mM Hepes pH 7.5, 500 mM NaCl, 0.5mM NaN3. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Do not freeze.
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Reconstitution Calculator
Background: GABARAP
gamma -Aminobutyric Acid Receptor-associated Protein (GABARAP), also known as Apg8p1, is 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the Autophagy-related 8 (Atg8) family of proteins (1). The GABARAP subfamily also includes GABARAPL1. GABARAP/Apg8p1 has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GABARAP/Apg8p1 was first described for its putative involvement GABA A Receptor trafficking (2,3). However, it is best known for its role in autophagy (1,4). GABARAP/Apg8p1 covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome maturation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).
- Shpilka, T. et al. (2011) Genome Biol. 12:226.
- Wang, H. et al. (1999) Nature 397:69.
- Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
- Weidberg, H. et al. (2010) EMBO J. 29:1792.
- Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.
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