Adipogen/Ubiquitin (K11 only Mutant) (human) (rec.) (His)/AG-40T-0468-M001/1 mg

来自 : 发布时间：2024-05-12

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UBB; Ubiquitin B

Protein

E. coli

Human ubiquitin K11 only mutant (Accession Nr. P0CG47) fused to a N-terminal His-tag. Except K11 all other lysines are mutated to arginine.

Human

Lyophilized.

Use: This ubiquitin mutant contains only a single lysine, K11, with all other lysines mutated to arginine. It renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules only via the K11 lysine. Typical concentrations for non rate-limiting support of in vitro conjugation reactions range from 0.2-1mM depending on experimental conditions.

Manufactured by Boston Biochem

BLUE ICE

+4°C

-20°C

Aliquot to avoid freeze/thaw cycles.

Stable for at least 1 year after receipt when stored at -20°C.

No

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Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting.